LOCUS       AB162195                 987 bp    DNA     linear   BCT 11-NOV-2004
DEFINITION  Pseudomonas fluorescens Pf-5 2-KGalARE gene for 2-ketogalactonate
            reductase, complete cds.
ACCESSION   AB162195
VERSION     AB162195.1  GI:55699941
KEYWORDS    .
SOURCE      Pseudomonas fluorescens Pf-5
  ORGANISM  Pseudomonas fluorescens Pf-5
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1
  AUTHORS   Tanimura,R., Hosaka,H., Matsuura,T., Nakagawa,A., Ikehara,K. and
            Iwamoto,R.
  TITLE     Purification, Characterization, and Crystallization of
            2-ketogalactonate reductase from Pseudomonas fluorescens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 987)
  AUTHORS   Tanimura,R., Hosaka,H., Matsuura,T., Nakagawa,A. and Iwamoto,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2004) Ryoko Iwamoto, Nara Women's University,
            Department of Chemistry, Faculty of Science; Kitauoya Nishimachi,
            Nara, Nara, 630-8506, Japan (E-mail:kurushima@cc.nara-wu.ac.jp,
            Tel:81-742-20-3403(ex.3043), Fax:81-742-20-3402)
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Pseudomonas fluorescens Pf-5"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:220664"
     gene            complement(1..987)
                     /gene="2-KGalARE"
     CDS             complement(1..987)
                     /gene="2-KGalARE"
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketogalactonate reductase"
                     /protein_id="BAD69623.1"
                     /db_xref="GI:55699942"
                     /translation="MKKHLVLYKKLSPTLMARLQEQAQVTLIETLDAAGLNALRQALP
                     SADGLLGASLRLDAELLDLAPRLRAVASVSVGVDNYDIDYLTQRRILLSNTPDVLTET
                     TADTGFALILATARRVVELANLVRAGQWQRNIGPAHFGTDVHGKTLGIIGMGRIGEAL
                     AQRGHFGFGMPLIYHSTRPKPAVEQRFNAQYRSLEQLLEEADFICLTLPLTERTQGLI
                     GAREFALMRPESIFINISRGKVVDEAALIEALQQRRIRGAGLDVFEREPLDHDSPLLQ
                     LPNVVATPHIGSATHETREAMARCAVDNLLAALAGQRPPNLVNPAAWTSPRP"
ORIGIN      
        1 ttaggggcgc gggctggtcc acgccgcggg gttgaccagg ttcggtgggc gctggccggc
       61 cagggccgcc agcaggttgt ccaccgcgca gcgggccatg gcttcgcggg tttcgtgggt
      121 ggccgagccg atgtgcgggg tggccacgac gttgggcagt tgcagcagcg gggagtcgtg
      181 gtccagcggc tcgcgctcga agacgtccag cccggcgccg cgaatccgcc gttgttgcag
      241 ggcttctatc agcgccgctt catccaccac cttgccccgg gaaatattga tgaagatgct
      301 ttcggggcgc atcagggcga actcccgggc gccgatcagg ccctgggtgc gttcggtcag
      361 gggcagggtc aggcagatga agtcggcttc ctcaagcagt tgctccaggc tccggtactg
      421 ggcattgaag cgttgctcca ccgcgggctt gggccgggtg ctgtggtaga tcagcggcat
      481 gccgaagccg aaatgcccgc gctgggccag ggcttcgccg atgcgcccca tgccgatgat
      541 gcccagggtc ttaccgtgga catccgtgcc aaagtgcgcc ggcccgatgt tgcgctgcca
      601 ttgcccggcg cgcaccaggt tggccagttc caccacccgc cgggcggtcg ccaggatcag
      661 cgcgaagccg gtgtcggcgg tggtctcggt gagcacgtcg ggggtgttgc tgagcaggat
      721 gcggcgctgg gtcaggtagt cgatgtcata gttgtcgacc cccaccgaaa cgctggccac
      781 cgcccgcaac cggggcgcca ggtccagcag ttcggcgtcc aggcgcaggc tggcgcccag
      841 cagcccgtcg gcgctcggca gcgcctggcg cagggcattc agccccgccg cgtcgagggt
      901 ttcgatcagg gttacctgcg cctgctcctg caggcgggcc atcagcgttg gggagagttt
      961 cttgtacagc accaggtgtt ttttcat
//