ID AB174850 standard; genomic DNA; PRO; 4966 BP. XX AC AB174850; XX SV AB174850.1 XX DT 23-SEP-2004 (Rel. 81, Created) DT 23-SEP-2004 (Rel. 81, Last updated, Version 1) XX DE Pseudomonas putida sfnB, sfnA, sfnF, sfnG genes for acyl-CoA DE dehydrogenase-family protein, acyl-CoA dehydrogenase-family protein, DE NADH-dependent FMN reductase, FMNH2-dependent dimethyl sulfone DE monooxygenase, complete cds. XX KW . XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-4966 RA Endoh T., Habe H., Nojiri H., Yamane H., Omori T.; RT ; RL Submitted (22-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Takayuki Endoh, The University of Tokyo, Biotechnology Research Center; RL Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan RL (E-mail:utendoh@mail.ecc.u-tokyo.ac.jp, Tel:81-3-5841-3062, RL Fax:81-3-5841-8030) XX RN [2] RA Endoh T., Habe H., Nojiri H., Yamane H., Omori T.; RT "The Pseudomonas putida sfnFG operon is required for dimethyl sulfone RT utilization and is regulated by a sigma54-dependent transcriptional RT activator, SfnR"; RL Unpublished. XX RN [3] RA Endoh T., Habe H., Yoshida T., Nojiri H., Omori T.; RT "A CysB-regulated and sigma54-dependent regulator, SfnR, is essential for RT dimethyl sulfone metabolism of Pseudomonas putida strain DS1"; RL Microbiology 149:991-1000(2003). XX RN [4] RX DOI; 10.1007/s00253-003-1233-7. RX PUBMED; 12835925. RA Endoh T., Kasuga K., Horinouchi M., Yoshida T., Habe H., Nojiri H., RA Omori T.; RT "Characterization and identification of genes essential for dimethyl RT sulfide utilization in Pseudomonas putida strain DS1"; RL Appl. Microbiol. Biotechnol. 62(1):83-91(2003). XX FH Key Location/Qualifiers FH FT source 1..4966 FT /db_xref="taxon:303" FT /mol_type="genomic DNA" FT /organism="Pseudomonas putida" FT /strain="DS1" FT CDS complement(170..1408) FT /codon_start=1 FT /db_xref="GOA:Q65YX2" FT /db_xref="UniProt/TrEMBL:Q65YX2" FT /transl_table=11 FT /gene="sfnB" FT /product="acyl-CoA dehydrogenase-family protein" FT /protein_id="BAD51727.1" FT /translation="MSEPHLQQRDESIVLHPSSKPAHRLGSEREAIEAAHALAQDFRRE FT AAARDRERRLPLAELEAYSQSGLWSITVPQAYGGLGASYRTVGEVFKIISAADGSLGQL FT PQNHFVILAHIGFDASDAQKRFFFDLALSGARFGNAFSERNGKHVADLQTRITRDGDDY FT VVNGQKFFSTGALVAHWIPIVSVDEQGRPHLAVVPRETEGLKVINDWSSFGQRTTASGT FT VLIEQVRVQASQVVPIWQAFDRPTAGGAISQFIQAAIDAGLAREALADTLDYVRRHTRP FT WIDSGLEKATDDPYILQQLGDLQIRLRAAEAVLDLAADAIDLAIREPSEESVAEASIRT FT AEAKVLTTEVAILASNRLFELAGTRSTLEEYSLDRHWRNARVHTLHDPVRWKYHLIGNY FT LLNGIKPPRHPWS" FT CDS complement(1524..2762) FT /codon_start=1 FT /db_xref="UniProt/TrEMBL:Q65YX1" FT /transl_table=11 FT /gene="sfnA" FT /product="acyl-CoA dehydrogenase-family protein" FT /protein_id="BAD51728.1" FT /translation="MTAQAHLPQLSIGTDYETLAARFRPIFARIAEGSVERERTRSLPF FT EPIRWLKEAGFGAVRVPVEHGGAGASLPQLFQLLIELAEADSNVPQALRGHFAFVEDRL FT NAHASAAQDVWFKRFVEGDLVGCAWTEVGSVKIGDVITRVSRKGDKWVVNGTKYYSTGS FT LFSDWIDLFARRDDTGGDVIAAIRTRQPGITQSDDWDGFGQRTTGSGTSVFENAEVEEE FT NIIDFATRFKYQTAFYQLVLLAVIVGSGRAALRDFSQETRKRTRVFSHGNAPAVSQDPQ FT VLQVIGKACALVYAAEAGTLRATEAAQQAYLARFGHDEAAERQANIAAELESAQAQVAA FT VELVLRATSDLFNTLGASSTSTTKQLDRHWRNARTAAVHNPVIYKERIIGDWHVNGTEP FT PYVWQIGGGAKQR" FT CDS 3102..3662 FT /codon_start=1 FT /db_xref="UniProt/TrEMBL:Q65YX0" FT /transl_table=11 FT /gene="sfnF" FT /product="NADH-dependent FMN reductase" FT /protein_id="BAD51729.1" FT /translation="MNTSVIRVVVVSGSLRAPSRTHGLLQALVEKLQARLSNLDVHWVR FT IAELCSALSGSLERDTASADLQLHLQAIEQADLLLVGSPVYRASYTGLFKHLFDLVDHQ FT SLRGVPVVLAATGGSERHALMIDHQLRPLFAFFQAHTLPYGLYASVEAFDQHHLVEPAQ FT FERIERVLDTVSAFFQIPVASAA" FT CDS 3694..4785 FT /codon_start=1 FT /db_xref="GOA:Q65YW9" FT /db_xref="UniProt/TrEMBL:Q65YW9" FT /transl_table=11 FT /gene="sfnG" FT /product="FMNH2-dependent dimethyl sulfone monooxygenase" FT /protein_id="BAD51730.1" FT /translation="MSQPIKFAYWVPNVSGGLVVSKIEQRTSWDIDYNRKLAQIAERSG FT FEYALSQIRFTAGYGADNQHESVTISHALLAATEKLKVIAAILPGPWSPALAAKQLATI FT DQFTGGRIAVNVVSGWFKGEFRAIGEPWLEHDERYRRSEEFIRALKGIWTQDNFSFHGD FT FYRFNDYTLKPKPLQRPHPEIFQGGSSRAARDMASRVSDWYFTNGNSVEGIKAQVDDIR FT AKAAANGHAVKIGVNAFVIARDTEEEARAVLAEIIAKADPEAVNGFGSEVKNAGAASPE FT GEGNWAKSTFEDLVQYNDGFKTNLIGTPRQIAERIVALKAIGVDLILSGFLHFQEEVEY FT FGRHVLPLVRELEQERRAAVAVA" XX SQ Sequence 4966 BP; 851 A; 1498 C; 1641 G; 976 T; 0 other; gcggccgccg agcggattgc acgggaagac atccggcatt tctacctggg cggcaaggcc 60 ggctgatggg gcggcggcat ctgcaaccgc cttgcatcat ccagcactga tgcttgttta 120 atgctccggg aaccccctgg ccgaatcctg cgcccggcca gggctgcgct cagctccagg 180 gatggcgcgg cggcttgatg ccgttgagca ggtagttgcc gatgaggtgg tatttccagc 240 gtacggggtc gtgcagagta tgcacgcggg cattacgcca gtgacggtca agggagtatt 300 cctccagggt cgagcgggtg cctgccaact cgaacaagcg attgctggca aggatggcca 360 cctcggtcgt gagcaccttg gcttcggccg tgcggatgga ggcctcggcc acgctctctt 420 cactgggttc gcgtattgcc aggtcgatgg catctgccgc cagatccagc acggcctcgg 480 cggcgcgcag gcgaatctgc aggtcgccga gttgctggag gatgtaaggg tcatcggtgg 540 ctttctccag tcccgaatcg atccagggac gagtgtgtcg gcgcacgtag tccagggtgt 600 cggccagcgc ttctcgtgcc aaccccgcat cgatggccgc ctggatgaac tgcgagatcg 660 ctccgccagc ggtagggcgg tcgaaggcct gccagattgg caccacttgg ctggcctgca 720 cccgcacctg ctcgatcagc accgtgccgc ttgccgtggt gcgctggccg aagctggacc 780 agtcgttgat caccttgagc ccttcggttt cccgtggcac cacggccaga tgcgggcgcc 840 cctgctcgtc cacgctgacg atgggaatcc agtgtgccac cagggcgccg gtggagaaga 900 atttctgccc gttgactacg tagtcgtcgc catcgcgtgt gatcctggtc tggaggtccg 960 cgacgtgctt gccattgcgt tcggagaagg cgttgccgaa gcgagctccg gacagggcaa 1020 ggtcgaagaa gaagcgcttc tgcgcgtcgc tggcgtcgaa gccgatgtgc gcgaggatga 1080 cgaagtgatt ctgcggtaac tggccgagtg agccgtcggc tgccgagatg atcttgaaca 1140 cctcgccgac ggtgcggtag ctggctccca ggcccccata agcctgaggc acggtgatgc 1200 tccagaggcc gctttgcgag taggcctcca gctccgccag gggcaggcgg cgttcgcggt 1260 cacgggcggc agcctcgcgg cggaagtcct gagccagggc gtgcgccgcc tcgatggctt 1320 cgcgctcact gcctaagcgg tgggctggtt tgctggatgg gtgcagcacg atgctttcat 1380 ctcgctgctg caggtggggt tcgctcatca aagggctcct ttagaaacgg tcgttgagct 1440 tgatgtcctg gatgaaaagt tacgccgacc cgcgagtgtg gctcgggggt gggcgtgatc 1500 gcagggggat tggaatggcg gcttcagcgc tgcttggcac caccgccgat ctgccagacg 1560 tagggcggct cggtgccatt gacgtgccag tcgccgatga tgcgttcctt gtagatcaca 1620 gggttgtgca cggcagcggt gcgcgcgttg cgccagtggc ggtcaagttg cttggtggta 1680 ctggtgctgg aggcgcccag ggtgttgaac aggtcgctgg tggcacgcag caccagctcc 1740 acggccgcca cctgcgcctg ggctgactcc agttcggcgg caatattggc ctggcgctcg 1800 gcggcctcgt catggccgaa gcgagccagg taggcttgct gcgcagcctc ggtggcgcgc 1860 agggtgccag cctctgcggc gtagaccagc gcgcaggcct tgccgatgac ctgcaacacc 1920 tgcggatcct ggcttaccgc tggtgcattg ccatggctga acacgcgcgt gcgtttgcgg 1980 gtttcctggc tgaagtcgcg cagggcggcg cgacccgaac cgacgatgac ggcgagcagt 2040 accagttggt agaaggcggt ctggtacttg aagcgggtgg cgaagtcgat gatgttctct 2100 tcctcgacct ctgcgttctc gaacaccgag gtgccactgc ctgtcgtgcg ttggccgaag 2160 ccgtcccagt catcgctctg ggtgatgcct ggctgtcggg tgcggatcgc cgcgatcaca 2220 tcaccaccgg tgtcgtcgcg gcgggcgaac aggtcgatcc agtcggagaa gaggctgccg 2280 gtgctgtagt acttggttcc gttgaccacc catttgtcgc ctttgcgcga gacacgggtg 2340 atgacatcgc caatcttcac cgaacccact tccgtccagg cgcaacccac cagatcacct 2400 tcgacgaagc gtttgaacca gacgtcctgc gctgcactgg cgtgggcatt gagacggtct 2460 tcgacgaaag cgaagtgccc gcgcaatgcc tgcggcacgt tggagtcggc ttcggccagt 2520 tcgatcagca gttggaacag ttgcggcaac gaggcgccag cgccgccatg ttcgacggga 2580 acacgcacgg cgccaaagcc ggcctccttg agccagcgga tgggctcgaa aggcaggctg 2640 cgggtgcgct cccgctccac gctaccttcg gcgatgcgcg caaagattgg ccggaagcgg 2700 gcggcaaggg tttcgtagtc ggttccgatg gacagttgag gcaggtgggc ttgagcggtc 2760 attgcagttc tcctcgatgg gtgcggttgc gcaccatcgc tccttcgcaa actccgtgcc 2820 ctttaaaaaa gtgcttttaa atcaaatgct tgatcatttt tcgttgacgc gcgggctgtg 2880 tcgctggcaa cagcctgtcg cagggctgtt gccaggcggt cattcggcgc ctcaggccct 2940 gctgttggct tgcagtcagt gattcaacag cctgcttgtg ccggagcagc aatggctgtt 3000 gagtgttttg gccaacgcat tgattttgaa cgctttattg gtttggcacg gtagctgctc 3060 aagagcttct ggacgaagcc gcgagggcag gagggatgtg catgaacaca agcgtcatca 3120 gggtcgtggt ggtatcgggc agcctgcgcg cgccatcgcg tactcatggg ttgctgcagg 3180 cgctggtgga gaagctgcag gcaaggttgt cgaaccttga cgtgcattgg gtgcgcatcg 3240 ccgagctttg cagcgccttg tccggctcgc ttgagcgcga taccgcctcc gccgatttac 3300 agcttcactt gcaggccatt gagcaggccg acctgttgtt ggtcggcagt ccggtttacc 3360 gcgcctccta cacgggtttg ttcaagcacc tgttcgatct ggtagaccac cagtcgctca 3420 gaggggtccc ggtagtgctg gcggccaccg gtggcagcga gcgccacgcc ctgatgatcg 3480 atcaccagtt acgccccctg ttcgccttct tccaggcgca caccctgccg tatggcttgt 3540 acgccagcgt tgaggccttc gaccagcacc atctggtcga gcccgcgcag ttcgaacgca 3600 tcgaacgggt gctcgacacc gtcagcgcgt tcttccagat cccggtcgcc agtgcggcct 3660 gacttcattt cccccggcaa caggagcatc gccatgagcc aaccaatcaa attcgcctac 3720 tgggtcccca atgtcagcgg cggcctcgtc gtcagcaaga ttgagcagcg caccagctgg 3780 gacatcgact acaaccgcaa gctggcacag atcgccgagc gttccgggtt cgagtacgcg 3840 ctgtcgcaga tccgttttac tgccggctac ggcgccgaca accagcacga gtcggtgacc 3900 atcagccatg cgctgctggc agccaccgag aagctcaagg tcatcgccgc aatcctgccc 3960 ggcccgtggt cgcccgcgct ggcggccaag caactggcga ccattgacca gttcaccggc 4020 ggacgcattg ccgtcaatgt ggtgtcaggc tggttcaaag gcgagtttcg cgccatcggc 4080 gagccttggc tggaacatga cgagcgctac cgtcgctccg aggagttcat ccgtgccctc 4140 aagggtatct ggacccagga caacttcagc ttccacggtg acttctaccg tttcaacgac 4200 tacaccctca agcccaagcc gctgcaacgg ccgcatccgg agattttcca gggcggcagc 4260 tcgcgggctg cgcgtgacat ggcatccagg gtctcggact ggtatttcac taacggcaac 4320 agcgtcgagg gtatcaaggc acaggtcgat gacatccgtg ccaaggccgc agccaacggg 4380 cacgcggtga agatcggggt aaacgctttc gtcattgccc gtgacaccga ggaagaagcc 4440 cgtgcggtat tggcagaaat catcgccaag gccgacccgg aggcggttaa cggcttcggc 4500 agcgaagtga agaacgcagg ggcggccagc ccggaaggcg aaggtaactg ggcaaagtcc 4560 accttcgagg acttggtgca gtacaacgat gggttcaaga ccaacctgat cggcacccca 4620 cggcagatcg ccgagcgcat cgtcgcgctc aaggccatcg gcgtcgacct gattctctcc 4680 ggcttcctgc atttccagga agaggtcgag tacttcggca gacacgtact gccgctggtg 4740 cgcgaactgg agcaggaaag gcgcgcagcc gtggccgtgg cctgaggccc gtcgccatgc 4800 atggggaacg tacagacaac ctgcccaagg tgtgggcggt ggtttgggct gtcgctggct 4860 tcgtcgggct ggtcgccggc tgctctgggg cgcacgcctg ggcgtgcagt tttacctctc 4920 gctggtggtg ctcaaagccc tgccggtgtt gatcatcggc ggccgc 4966 //