ID   GUN5_THEFU     STANDARD;      PRT;   466 AA.
AC   Q01786;
DT   01-JUL-1993 (Rel. 26, Created)
DT   15-JUL-1999 (Rel. 38, Last sequence update)
DT   25-OCT-2004 (Rel. 45, Last annotation update)
DE   Endoglucanase E-5 precursor (EC 3.2.1.4) (Endo-1,4-beta-glucanase E-4)
DE   (Cellulase E-5) (Cellulase E5).
GN   Name=celE;
OS   Thermomonospora fusca.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptosporangineae; Nocardiopsaceae; Thermobifida.
OX   NCBI_TaxID=2021;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=YX;
RX   MEDLINE=91258320; PubMed=1904434;
RA   Lao G., Ghangas G.S., Jung E.D., Wilson D.B.;
RT   "DNA sequences of three beta-1,4-endoglucanase genes from
RT   Thermomonospora fusca.";
RL   J. Bacteriol. 173:3397-3407(1991).
RN   [2]
RP   SEQUENCE REVISION.
RA   Lao G., Ghangas G.S., Jung E.D., Wilson D.B.;
RL   Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 137-142 AND 157-166.
RC   STRAIN=YX;
RA   Irwin D.C., Spezio M., Walker L.P., Wilson D.B.;
RT   "Activity studies of eight purified cellulases: specificity,
RT   synergism, and binding domain effects.";
RL   Biotechnol. Bioeng. 42:1002-1013(1993).
CC   -!- CATALYTIC ACTIVITY: Endohydrolysis of 1,4-beta-D-glucosidic
CC       linkages in cellulose, lichenin and cereal beta-D-glucans.
CC   -!- PATHWAY: Cellulose degradation.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A)
CC       family.
CC   --------------------------------------------------------------------------
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CC   between  the Swiss Institute of Bioinformatics  and the  EMBL outstation -
CC   the European Bioinformatics Institute.  There are no  restrictions on  its
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DR   EMBL; L01577; AAC09379.1; -.
DR   PIR; C42360; C42360.
DR   HSSP; Q59232; 1LF1.
DR   InterPro; IPR001919; Bac_celose-bind.
DR   InterPro; IPR008965; Cellul_bind.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   Pfam; PF00553; CBM_2; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SMART; SM00637; CBD_II; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
KW   Cellulose degradation; Direct protein sequencing; Glycosidase;
KW   Hydrolase; Signal.
FT   SIGNAL        1     36
FT   CHAIN        37    466       Endoglucanase E-5.
FT   ACT_SITE    299    299       Proton donor (By similarity).
FT   ACT_SITE    391    391       Nucleophile (By similarity).
SQ   SEQUENCE   466 AA;  49801 MW;  1CF0ADFBF2DEF82E CRC64;
     MAKSPAARKG XPPVAVAVTA ALALLIALLS PGVAQAAGLT ATVTKESSWD NGYSASVTVR
     NDTSSTVSQW EVVLTLPGGT TVAQVWNAQH TSSGNSHTFT GVSWNSTIPP GGTASSGFIA
     SGSGEPTHCT INGAPCDEGS EPGGPGGPGT PSPDPGTQPG TGTPVERYGK VQVCGTQLCD
     EHGNPVQLRG MSTHGIQWFD HCLTDSSLDA LAYDWKADII RLSMYIQEDG YETNPRGFTD
     RMHQLIDMAT ARGLYVIVDW HILTPGDPHY NLDRAKTFFA EIAQRHASKT NVLYEIANEP
     NGVSWASIKS YAEEVIPVIR QRDPDSVIIV GTRGWSSLGV SEGSGPAEIA ANPVNASNIM
     YAFHFYAASH RDNYLNALRE ASELFPVFVT EFGTETYTGD GANDFQMADR YIDLMAERKI
     GWTKWNYSDD FRSGAVFQPG TCASGGPWSG SSLKASGQWV RSKLQS
//