ID   G3P2_TRIKO     STANDARD;      PRT;   337 AA.
AC   P17730;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 2.
DT   04-APR-2006, entry version 52.
DE   Glyceraldehyde-3-phosphate dehydrogenase 2 (EC 1.2.1.12) (GAPDH2).
GN   Name=gpd2;
OS   Trichoderma koningii.
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
OX   NCBI_TaxID=55202;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=M3947;
RX   MEDLINE=93176825; PubMed=8439569; DOI=10.1016/0167-4781(93)90267-H;
RA   Watanabe H., Hasumi K., Fukushima Y., Sakai K., Endo A.;
RT   "Cloning of two isozymes of Trichoderma koningii
RT   glyceraldehyde-3-phosphate dehydrogenase with different sensitivity to
RT   koningic acid.";
RL   Biochim. Biophys. Acta 1172:43-48(1993).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-31.
RC   STRAIN=M3947;
RX   MEDLINE=91031446; PubMed=2226438;
RA   Sakai K., Hasumi K., Endo A.;
RT   "Two glyceraldehyde-3-phosphate dehydrogenase isozymes from the
RT   koningic acid (heptelidic acid) producer Trichoderma koningii.";
RL   Eur. J. Biochem. 193:195-202(1990).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- ENZYME REGULATION: Inhibited by koningic acid through the
CC       interaction of cysteine residues with koningic acid even at very
CC       low concentrations.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from
CC       D-glyceraldehyde 3-phosphate: step 1.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: This protein is a koningic acid (antibiotic)-
CC       sensitive GAPDH isozyme. It is present only when no antibiotic is
CC       produced.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; D14518; BAA03391.1; -; mRNA.
DR   HSSP; P56649; 1CRW.
DR   InterPro; IPR000173; GAP_DH.
DR   InterPro; IPR006424; GAPDH_I.
DR   PANTHER; PTHR10836; GAP_dhdrogenase; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
KW   Direct protein sequencing; Glycolysis; NAD; Oxidoreductase.
FT   INIT_MET      0      0
FT   CHAIN         1    337       Glyceraldehyde-3-phosphate dehydrogenase
FT                                2.
FT                                /FTId=PRO_0000145586.
FT   NP_BIND      12     13       NAD (By similarity).
FT   REGION      150    152       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      210    211       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    151    151       Nucleophile (By similarity).
FT   BINDING      34     34       NAD (By similarity).
FT   BINDING      79     79       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     181    181       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     233    233       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     315    315       NAD (By similarity).
FT   SITE        178    178       Activates thiol group during catalysis
FT                                (By similarity).
FT   CONFLICT     24     24       H -> K (in Ref. 2).
FT   CONFLICT     26     26       D -> N (in Ref. 2).
SQ   SEQUENCE   337 AA;  35975 MW;  098FA3C4BF56C615 CRC64;
     APIKVGINGF GRIGRIVFRN AVEHPDIEVV AVNDPFIETT YAAYMLKYDS SHGLFKGEVE
     VDGKDLVVNG KKVRFYTERN PADIKWSETG AEYVVESTGV FTTTEKAKAH LVGGAKKVII
     SAPSADAPMY VMGVNESDYD GSADVISNAS CTTNCLAPLA KVINDNYGIV EGLMTTVHSY
     TATQKTVDGP SAKDWRGGRG AAQNIIPSST GAAKAVGKVI PALNGKLTGM SIRVPTANVS
     VVDLTVRIEK GASYEEITET IKKAADGPLK GVLAYTGDDV VSSDMLGNTN SSIFDIKAGI
     SLNKNFVKLV SWYDNEWGYS RRVLDLLAHV AKVDASK
//