ID   RNT1_TRIHA     STANDARD;      PRT;   106 AA.
AC   P26875;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   07-FEB-2006, entry version 29.
DE   Guanyl-specific ribonuclease Th1 (EC 3.1.27.3) (RNase Th1).
OS   Trichoderma harzianum (Hypocrea lixii).
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
OX   NCBI_TaxID=5544;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=01;
RX   MEDLINE=88340050; PubMed=3139001;
RA   Bezborodova S.I., Vasileva-Tonkova E.S., Polyakov K.M.,
RA   Shlyapnikov S.V.;
RT   "Isolation, analysis of amino acid sequence and crystallization of the
RT   extracellular ribonuclease Th1 from Trichoderma harzianum-01.";
RL   Bioorg. Khim. 14:453-466(1988).
CC   -!- CATALYTIC ACTIVITY: Two-stage endonucleolytic cleavage to
CC       nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in
CC       G-P with 2',3'-cyclic phosphate intermediates.
CC   -!- SIMILARITY: Belongs to the ribonuclease N1/T1 family.
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DR   PIR; JN0428; JN0428.
DR   HSSP; P00651; 1RN1.
DR   InterPro; IPR000026; Ribonuc_N1T1.
DR   Pfam; PF00545; Ribonuclease; 1.
KW   Direct protein sequencing; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN         1    106       Guanyl-specific ribonuclease Th1.
FT                                /FTId=PRO_0000137375.
FT   ACT_SITE     39     39       By similarity.
FT   ACT_SITE     58     58       Proton acceptor (By similarity).
FT   ACT_SITE     92     92       Proton donor (By similarity).
FT   DISULFID      5    103       By similarity.
FT   DISULFID     23     84       Probable.
SQ   SEQUENCE   106 AA;  10750 MW;  892A8BFE043AA022 CRC64;
     DTATCGKVFY SASAVSAASN AACNYVRAGS TAGGSTYPHV YNNYEGFRFK GLSKPFYEFP
     ILSSGKTYTG GSPGADRVVI NGQCSIAGII THTGASGNAF VACGGT
//