ID   CHI4_TRIHA     STANDARD;      PRT;   423 AA.
AC   P48827;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   07-FEB-2006, entry version 45.
DE   42 kDa endochitinase precursor (EC 3.2.1.14).
GN   Name=chit42;
OS   Trichoderma harzianum (Hypocrea lixii).
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
OX   NCBI_TaxID=5544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 35-52; 93-107;
RP   371-385 AND 397-414.
RX   MEDLINE=95269313; PubMed=7750151; DOI=10.1007/BF00326583;
RA   Garcia I., Lora J.M., de la Cruz J., Benitez T., Llobell A.,
RA   Pintor-Toro J.A.;
RT   "Cloning and characterization of a chitinase (chit42) cDNA from the
RT   mycoparasitic fungus Trichoderma harzianum.";
RL   Curr. Genet. 27:83-89(1994).
CC   -!- FUNCTION: Morphogenetic role during apical growth, cell division
CC       and differentiation (cell wall morphogenesis). Antifungal agent.
CC   -!- CATALYTIC ACTIVITY: Random hydrolysis of N-acetyl-beta-D-
CC       glucosaminide 1,4-beta-linkages in chitin and chitodextrins.
CC   -!- SUBCELLULAR LOCATION: Secreted protein.
CC   -!- INDUCTION: Specifically induced by chitin and is catabolite
CC       repressed.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase
CC       class II subfamily.
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DR   EMBL; S78423; AAB34355.1; -; mRNA.
DR   PIR; S51369; S51369.
DR   HSSP; P54196; 1LL7.
DR   InterPro; IPR011583; Chitinase_II.
DR   InterPro; IPR001223; Glyco_hydro_18.
DR   InterPro; IPR001579; Glyco_hydro_18AS.
DR   Pfam; PF00704; Glyco_hydro_18; 1.
DR   ProDom; PD000471; Chitinase_II; 1.
DR   SMART; SM00636; Glyco_18; 1.
DR   PROSITE; PS01095; CHITINASE_18; 1.
KW   Carbohydrate metabolism; Chitin degradation; Chitin-binding;
KW   Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Signal; Zymogen.
FT   SIGNAL        1     22       Potential.
FT   PROPEP       23     34
FT                                /FTId=PRO_0000011934.
FT   CHAIN        35    423       42 kDa endochitinase.
FT                                /FTId=PRO_0000011935.
FT   ACT_SITE    171    171       Proton donor (By similarity).
FT   CARBOHYD    218    218       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   423 AA;  46057 MW;  DF90378EED1C30BE CRC64;
     MLSFLGKSVA LLAALQATLS SPKPGHRRAS VEKRANGYAN SVYFTNWGIY DRNFQPADLV
     ASDVTHVIYS FMNLQADGTV ISGDTYADYE KHYADDSWND VGTNAYGCVK QLFKVKKANR
     GLKVLLSIGG WTWSTNFPSA ASTDANRKNF AKTAITFMKD WGFDGIDIDW EYPADATQAS
     NMILLLKEVR SQRDAYAAQY APGYHFLLTI AAPAGKDNYS KLRLADLGQV LDYINLMAYD
     YAGSFSPLTG HDANLFNNPS NPNATPFNTD SAVKDYINGG VPANKIVLGM PIYGRSFQNT
     AGIGQTYNGV GSGSWEAGIW DYKALPKAGA TVQYDSVAKG YYSYNSATKE LISFDTPDMI
     NTKVAYLKSL GLGGSMFWEA SADKKGADSV IGTSHRALGG LDTTQNLLSY PNSKYDNIKN
     GLN
//