ID   GUX1_TRIKO     STANDARD;      PRT;   513 AA.
AC   P62695; P00725;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   21-MAR-2006, entry version 16.
DE   Exoglucanase 1 precursor (EC 3.2.1.91) (Exoglucanase I)
DE   (Exocellobiohydrolase I) (CBHI) (1,4-beta-cellobiohydrolase).
GN   Name=cbh1;
OS   Trichoderma koningii.
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
OX   NCBI_TaxID=55202;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=G-39;
RX   MEDLINE=94100788; PubMed=7764306;
RA   Wey T.T., Hseu T.H., Huang L.;
RT   "Molecular cloning and sequence analysis of the cellobiohydrolase I
RT   gene from Trichoderma koningii G-39.";
RL   Curr. Microbiol. 28:31-39(1994).
CC   -!- FUNCTION: The biological conversion of cellulose to glucose
CC       generally requires three types of hydrolytic enzymes: (1)
CC       Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2)
CC       Exocellobiohydrolases that cut the dissaccharide cellobiose from
CC       the nonreducing end of the cellulose polymer chain; (3)
CC       Beta-1,4-glucosidases which hydrolyze the cellobiose and other
CC       short cello-oligosaccharides to glucose.
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of 1,4-beta-D-glucosidic linkages
CC       in cellulose and cellotetraose, releasing cellobiose from the non-
CC       reducing ends of the chains.
CC   -!- SUBCELLULAR LOCATION: Secreted protein.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 7 (cellulase C)
CC       family.
CC   -!- SIMILARITY: Contains 1 CBM1 (fungal-type carbohydrate-binding)
CC       domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X69976; CAA49596.1; -; Genomic_DNA.
DR   SMR; P62695; 18-451.
DR   InterPro; IPR000254; CBD_fun.
DR   InterPro; IPR008985; ConA_like_lec_gl.
DR   InterPro; IPR001722; Glyco_hydro_7.
DR   Pfam; PF00734; CBM_1; 1.
DR   Pfam; PF00840; Glyco_hydro_7; 1.
DR   PRINTS; PR00734; GLHYDRLASE7.
DR   ProDom; PD001821; CBD_fungal; 1.
DR   ProDom; PD186135; Glyco_hydro_7; 1.
DR   SMART; SM00236; fCBD; 1.
DR   PROSITE; PS00562; CBM1_1; 1.
DR   PROSITE; PS51164; CBM1_2; 1.
KW   Carbohydrate metabolism; Cellulose degradation; Glycoprotein;
KW   Glycosidase; Hydrolase; Polysaccharide degradation;
KW   Pyrrolidone carboxylic acid; Signal.
FT   SIGNAL        1     17       By similarity.
FT   CHAIN        18    513       Exoglucanase 1.
FT                                /FTId=PRO_0000007926.
FT   DOMAIN      477    513       CBM1.
FT   REGION       18    453       Catalytic.
FT   REGION      454    477       Linker.
FT   ACT_SITE    143    143       By similarity.
FT   ACT_SITE    229    229       Nucleophile (By similarity).
FT   ACT_SITE    234    234       Proton donor (By similarity).
FT   MOD_RES      18     18       Pyrrolidone carboxylic acid (By
FT                                similarity).
FT   CARBOHYD     62     62       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD     81     81       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    287    287       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    401    401       N-linked (GlcNAc...) (Potential).
FT   DISULFID     21     89       By similarity.
FT   DISULFID     36     42       By similarity.
FT   DISULFID     67     88       By similarity.
FT   DISULFID     78     84       By similarity.
FT   DISULFID    155    414       By similarity.
FT   DISULFID    189    227       By similarity.
FT   DISULFID    193    226       By similarity.
FT   DISULFID    247    273       By similarity.
FT   DISULFID    255    260       By similarity.
FT   DISULFID    278    348       By similarity.
FT   DISULFID    485    502       By similarity.
FT   DISULFID    496    512       By similarity.
SQ   SEQUENCE   513 AA;  54073 MW;  9F5C0A8A854F2C12 CRC64;
     MYRKLAVISA FLATARAQSA CTLQSETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA
     TNSSTNCYDG NTWSSTLCPD NETCAKNCCL DGAAYASTYG VTTSGNSLSI GFVTQSAQKN
     VGARLYLMAS DTTYQEFTLL GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA
     GAKYGTGYCD SQCPRDLKFI NGQANVEGWE PSSNNANTGI GGHGSCCSEM DIWEANSISE
     ALTPHPCTTV GQEICEGDGC GGTYSDNRYG GTCDPDGCDW NPYRLGNTSF YGPGSSFTLD
     TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGSYSG NELNDDYCTA EEAEFGGSSF
     SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV
     PAQVESQSPN AKVTFSNIKF GPIGSTGNPS GGNPPGGNRG TTTTRRPATT TGSSPGPTQS
     HYGQCGGIGY SGPTVCASGT TCQVLNPYYS QCL
//