ID   ALP_TRIHA      STANDARD;      PRT;   409 AA.
AC   Q03420;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   21-MAR-2006, entry version 44.
DE   Alkaline proteinase precursor (EC 3.4.21.-) (ALP).
GN   Name=prb1;
OS   Trichoderma harzianum (Hypocrea lixii).
OC   Eukaryota; Fungi; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Hypocrea.
OX   NCBI_TaxID=5544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=IMI 206040;
RX   MEDLINE=93316857; PubMed=8326868;
RA   Geremia R.A., Goldman G.H., Jacobs D., Ardiles W., Vila S.B.,
RA   van Montagu M., Herrera-Estrella A.;
RT   "Molecular characterization of the proteinase-encoding gene, prb1,
RT   related to mycoparasitism by Trichoderma harzianum.";
RL   Mol. Microbiol. 8:603-613(1993).
CC   -!- FUNCTION: Serine protease, secreted specifically during the
CC       mycoparasitic process, which is involved in the degradation of
CC       phytopathogen cell walls, membranes and of the proteins released
CC       after lysis of the host.
CC   -!- SUBCELLULAR LOCATION: Secreted protein.
CC   -!- INDUCTION: By mycelia, fungal cell walls, and chitin, but only in
CC       the absence of glucose.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; M87518; AAA34211.1; -; Genomic_DNA.
DR   EMBL; M87516; AAA34209.1; -; mRNA.
DR   PIR; S32905; S32905.
DR   HSSP; Q99405; 1MPT.
DR   MEROPS; S08.066; -.
DR   InterPro; IPR000209; Pept_S8_S53.
DR   InterPro; IPR009020; Prot_inh_propept.
DR   InterPro; IPR010259; Prot_inh_S8A.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   Pfam; PF05922; Subtilisin_N; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
KW   Direct protein sequencing; Glycoprotein; Hydrolase; Protease;
KW   Serine protease; Signal; Zymogen.
FT   SIGNAL        1     20       Potential.
FT   PROPEP       21    120       Potential.
FT                                /FTId=PRO_0000026990.
FT   CHAIN       121    409       Alkaline proteinase.
FT                                /FTId=PRO_0000026991.
FT   ACT_SITE    161    161       Charge relay system (By similarity).
FT   ACT_SITE    192    192       Charge relay system (By similarity).
FT   ACT_SITE    353    353       Charge relay system (By similarity).
FT   CARBOHYD    252    252       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   409 AA;  42271 MW;  D0F548EA236C5E63 CRC64;
     MTSIRRLALY LGALLPAVLA APAALHKKPE AVPNKFIVTL KEGASIDTDS HLAWVTDIHT
     RSLTKRSTAG VEKTYNIHTW NAYAGEFDEE TIEQIKSNPD VASVEPDYIM HLSDIVEDKR
     ALTTQSGAPW GLGTVSHRTS GSTSYIYDSS AGAGTFAYVV DSGINTSHQQ FGGRASLGYN
     AAGGQHVDTL GHGTHVSGTI GGSTYGVAKQ ASLISVKVFA GESASTSVIL DGYNWAVNDI
     VSKSRASKSA INMSLGGPAS STWTTAINAA FNQGVLTIVA AGNGDSLGNP QPVSGTSPAN
     VPNAITVAAL DINWRTASFT NYGAGVDVFA PGVNILSSWI GSNTATNTIS GTSMATPHVV
     GLALYLQSLE GLTSPTAVTN RIKALATSGR VTGSLNGSPN VIIFNGNSS
//