ID   ERF4_TOBAC     STANDARD;      PRT;   225 AA.
AC   Q40477;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   07-FEB-2006, entry version 36.
DE   Ethylene-responsive transcription factor 4 (Ethylene-responsive
DE   element-binding factor 4 homolog) (EREBP-3) (NtERF3).
GN   Name=ERF4; Synonyms=ERF3, ERF-3;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=cv. Bright Yellow 4; TISSUE=Leaf;
RX   MEDLINE=95276459; PubMed=7756828; DOI=10.1105/tpc.7.2.173;
RA   Ohme-Takagi M., Shinshi H.;
RT   "Ethylene-inducible DNA binding proteins that interact with an
RT   ethylene responsive element.";
RL   Plant Cell 7:173-182(1995).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RX   DOI=10.1046/j.1365-313x.1998.00243.x;
RA   Suzuki K., Suzuki N., Ohme-Takagi M., Shinshi H.;
RT   "Immediate early induction of mRNAs for ethylene-responsive
RT   transcription factors in tobacco leaf strips after cutting.";
RL   Plant J. 15:657-665(1998).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RX   MEDLINE=20117508; PubMed=10652129;
RX   DOI=10.1046/j.1365-313X.1999.00634.x;
RA   Yamamoto S., Suzuki K., Shinshi H.;
RT   "Elicitor-responsive, ethylene-independent activation of GCC box-
RT   mediated transcription that is regulated by both protein
RT   phosphorylation and dephosphorylation in cultured tobacco cells.";
RL   Plant J. 20:571-579(1999).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   MEDLINE=20252856; PubMed=10792818;
RX   DOI=10.1046/j.1365-313x.2000.00709.x;
RA   Ohta M., Ohme-Takagi M., Shinshi H.;
RT   "Three ethylene-responsive transcription factors in tobacco with
RT   distinct transactivation functions.";
RL   Plant J. 22:29-38(2000).
RN   [5]
RP   TISSUE SPECIFICITY.
RX   MEDLINE=21333211; PubMed=11440157; DOI=10.1271/bbb.65.1270;
RA   Koyama T., Kitajima S., Sato F.;
RT   "Expression of PR-5d and ERF genes in cultured tobacco cells and their
RT   NaCl stress-response.";
RL   Biosci. Biotechnol. Biochem. 65:1270-1273(2001).
RN   [6]
RP   INDUCTION.
RX   MEDLINE=22084990; PubMed=12090623; DOI=10.1023/A:1015553232309;
RA   Nishiuchi T., Suzuki K., Kitajima S., Sato F., Shinshi H.;
RT   "Wounding activates immediate early transcription of genes for ERFs in
RT   tobacco plants.";
RL   Plant Mol. Biol. 49:473-482(2002).
RN   [7]
RP   FUNCTION, PROBABLE UBIQUITINATION, AND INTERACTION WITH UBC2.
RX   MEDLINE=22541787; PubMed=12654868; DOI=10.1093/jxb/erg136;
RA   Koyama T., Okada T., Kitajima S., Ohme-Takagi M., Shinshi H., Sato F.;
RT   "Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast
RT   two-hybrid system with tobacco ERF3 as bait and its characterization
RT   of specific interaction.";
RL   J. Exp. Bot. 54:1175-1181(2003).
CC   -!- FUNCTION: Acts as a transcriptional repressor. Binds to the GCC-
CC       box pathogenesis-related promoter element. Involved in the
CC       regulation of gene expression by stress factors and by components
CC       of stress signal transduction pathways mediated by ethylene, that
CC       seems to depend on a protein kinase/phosphatase cascade, and to be
CC       influenced by methyl-jasmonate. May regulate other AtERFs (By
CC       similarity).
CC   -!- SUBUNIT: Interacts with UBC2.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, in cultured cells and at
CC       low levels in buds and leaves. Highly expressed in cell culture,
CC       especially during the exponential phase.
CC   -!- INDUCTION: Strongly induced by ethephon (ethylene-releasing
CC       compound) in buds and to lower extent in leaves. Strongly induced
CC       by cycloheximide and mechanical stimuli. Wounding leads to a both
CC       local and systemical transient expression, independently of
CC       ethylene, and through a de-novo-protein-synthesis-independent
CC       regulation. Not influenced by methyl-jasmonate. Induction by
CC       purified xylanase from Trichoderma viride (TvX) and another
CC       elicitor from Phytophthora infestans (PiE), that appears to be
CC       mediated by a protein kinase cascade, and to be negatively
CC       regulated by protein phosphatases.
CC   -!- DOMAIN: The AP2/ERF domain binds specifically to the 5'-GCCGCC-3'
CC       motif. The affinity of this binding is higher if the seventh
CC       amino-acid of this domain is basic (By similarity).
CC   -!- DOMAIN: Contains a slightly degenerated ERF-associated amphiphilic
CC       repression (EAR) motif, which may be involved in the activity of
CC       transcriptional repression (By similarity).
CC   -!- PTM: Probably negatively regulated by UBC2, that targets it to the
CC       ubiquitin proteasome pathway after ubiquitination and leads it to
CC       proteolysis (Probable).
CC   -!- SIMILARITY: Belongs to the ethylene-response factor family. Class
CC       2 subfamily.
CC   -!- SIMILARITY: Contains 1 AP2/ERF DNA-binding domain.
CC   -!- CAUTION: Was named ERF3 but it corresponds to Arabidopsis ERF4.
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DR   EMBL; D38124; BAA07322.1; -; mRNA.
DR   PIR; T02433; T02433.
DR   HSSP; O80337; 2GCC.
DR   SMR; Q40477; 26-83.
DR   TRANSFAC; T02655; -.
DR   InterPro; IPR001471; TF_ERF.
DR   Pfam; PF00847; AP2; 1.
DR   PRINTS; PR00367; ETHRSPELEMNT.
DR   ProDom; PD001423; TF_ERF; 1.
DR   SMART; SM00380; AP2; 1.
DR   PROSITE; PS51032; AP2_ERF; 1.
KW   DNA-binding; Ethylene signaling pathway; Nuclear protein;
KW   Plant defense; Repressor; Transcription; Transcription regulation;
KW   Ubl conjugation.
FT   CHAIN         1    225       Ethylene-responsive transcription factor
FT                                4.
FT                                /FTId=PRO_0000112556.
FT   DNA_BIND     26     83       AP2/ERF.
FT   REGION       92    196       Interaction with UBC2.
FT   MOTIF       215    221       EAR-like (transcriptional repression)
FT                                (Potential).
SQ   SEQUENCE   225 AA;  24262 MW;  BFF148E5B0B4EFAE CRC64;
     MAVKNKVSNG NLKGGNVKTD GVKEVHYRGV RKRPWGRYAA EIRDPGKKSR VWLGTFDTAE
     EAAKAYDTAA REFRGPKAKT NFPSPTENQS PSHSSTVESS SGENGVHAPP HAPLELDLTR
     RLGSVAADGG DNCRRSGEVG YPIFHQQPTV AVLPNGQPVL LFDSLWRAGV VNRPQPYHVT
     PMGFNGVNAG VGPTVSDSSS AVEENQYDGK RGIDLDLNLA PPMEF
//