ID   ERF5_TOBAC     STANDARD;      PRT;   291 AA.
AC   Q40478;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   07-FEB-2006, entry version 36.
DE   Ethylene-responsive transcription factor 5 (Ethylene-responsive
DE   element-binding factor 5 homolog) (EREBP-4) (NtERF4).
GN   Name=ERF5; Synonyms=ERF4, ERF-4;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=cv. Bright Yellow 4; TISSUE=Leaf;
RX   MEDLINE=95276459; PubMed=7756828; DOI=10.1105/tpc.7.2.173;
RA   Ohme-Takagi M., Shinshi H.;
RT   "Ethylene-inducible DNA binding proteins that interact with an
RT   ethylene responsive element.";
RL   Plant Cell 7:173-182(1995).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RX   DOI=10.1046/j.1365-313x.1998.00243.x;
RA   Suzuki K., Suzuki N., Ohme-Takagi M., Shinshi H.;
RT   "Immediate early induction of mRNAs for ethylene-responsive
RT   transcription factors in tobacco leaf strips after cutting.";
RL   Plant J. 15:657-665(1998).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RX   MEDLINE=20117508; PubMed=10652129;
RX   DOI=10.1046/j.1365-313X.1999.00634.x;
RA   Yamamoto S., Suzuki K., Shinshi H.;
RT   "Elicitor-responsive, ethylene-independent activation of GCC box-
RT   mediated transcription that is regulated by both protein
RT   phosphorylation and dephosphorylation in cultured tobacco cells.";
RL   Plant J. 20:571-579(1999).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   MEDLINE=20252856; PubMed=10792818;
RX   DOI=10.1046/j.1365-313x.2000.00709.x;
RA   Ohta M., Ohme-Takagi M., Shinshi H.;
RT   "Three ethylene-responsive transcription factors in tobacco with
RT   distinct transactivation functions.";
RL   Plant J. 22:29-38(2000).
RN   [5]
RP   TISSUE SPECIFICITY.
RX   MEDLINE=21333211; PubMed=11440157; DOI=10.1271/bbb.65.1270;
RA   Koyama T., Kitajima S., Sato F.;
RT   "Expression of PR-5d and ERF genes in cultured tobacco cells and their
RT   NaCl stress-response.";
RL   Biosci. Biotechnol. Biochem. 65:1270-1273(2001).
RN   [6]
RP   INDUCTION.
RX   MEDLINE=22084990; PubMed=12090623; DOI=10.1023/A:1015553232309;
RA   Nishiuchi T., Suzuki K., Kitajima S., Sato F., Shinshi H.;
RT   "Wounding activates immediate early transcription of genes for ERFs in
RT   tobacco plants.";
RL   Plant Mol. Biol. 49:473-482(2002).
CC   -!- FUNCTION: Acts as a transcriptional activator. Binds to the GCC-
CC       box pathogenesis-related promoter element. Involved in the
CC       regulation of gene expression by stress factors and by components
CC       of stress signal transduction pathways mediated by ethylene, that
CC       seems to depend on a protein kinase/phosphatase cascade, and to be
CC       influenced by methyl-jasmonate.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Expressed in roots. Not expressed in cell
CC       culture.
CC   -!- INDUCTION: Strongly induced by ethephon (ethylene-releasing
CC       compound) in buds and in leaves. Also strongly induced by
CC       cycloheximide, mechanical stimuli. Wounding leads to a both local
CC       and systemic expression, independently of ethylene, and through a
CC       de-novo-protein-synthesis-independent regulation. Wound induction
CC       is reduced by methyl-jasmonate. Induction by purified xylanase
CC       from Trichoderma viride (TvX) and another elicitor from
CC       Phytophthora infestans (PiE), that appears to be mediated by a
CC       protein kinase cascade, and to be negatively regulated by protein
CC       phosphatases.
CC   -!- DOMAIN: The AP2/ERF domain binds specifically to the 5'-GCCGCC-3'
CC       motif. The affinity of this binding is higher if the seventh
CC       amino-acid of this domain is basic (By similarity).
CC   -!- SIMILARITY: Belongs to the ethylene-response factor family. Class
CC       3 subfamily.
CC   -!- SIMILARITY: Contains 1 AP2/ERF DNA-binding domain.
CC   -!- CAUTION: Was named ERF4 but it corresponds to Arabidopsis ERF5.
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DR   EMBL; D38125; BAA07323.1; -; mRNA.
DR   PIR; T02434; T02434.
DR   HSSP; O80337; 2GCC.
DR   SMR; Q40478; 143-205.
DR   TRANSFAC; T02656; -.
DR   InterPro; IPR001471; TF_ERF.
DR   Pfam; PF00847; AP2; 1.
DR   PRINTS; PR00367; ETHRSPELEMNT.
DR   ProDom; PD001423; TF_ERF; 1.
DR   SMART; SM00380; AP2; 1.
DR   PROSITE; PS51032; AP2_ERF; 1.
KW   Activator; DNA-binding; Ethylene signaling pathway; Nuclear protein;
KW   Plant defense; Transcription; Transcription regulation.
FT   CHAIN         1    291       Ethylene-responsive transcription factor
FT                                5.
FT                                /FTId=PRO_0000112565.
FT   DNA_BIND    144    202       AP2/ERF.
FT   COMPBIAS     36     44       Ser-rich.
SQ   SEQUENCE   291 AA;  32878 MW;  13E7ED8C819D5F48 CRC64;
     MASPQENCTT LDLIRQHLLD DNVFMEHYCP QPILYSQSSS SSESLNSIAS ELNNETFSFE
     PTLKYADTAQ SSNLDISSFF NNSKTEFDSF EFETKPNVSA ARISSNSPKQ TSFKERKPSL
     NIAIPMKQQE VVQKVEVVPT EKKHYRGVRQ RPWGKFAAEI RDPNRKGTRV WLGTFDTAIE
     AAKAYDRAAF KLRGSKAIVN FPLEVANFKQ QDNEILQPAN SGRKRMRETE NEEIVIKKEV
     KREERVPAAA APLTPSSWSA IWEGEDGKGI FEVPPLSPLS PHMAYSQLVM I
//