ID   ERF2_TOBAC     STANDARD;      PRT;   233 AA.
AC   Q40479;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   07-FEB-2006, entry version 36.
DE   Ethylene-responsive transcription factor 2 (Ethylene-responsive
DE   element-binding factor 2) (EREBP-2) (NtERF2).
GN   Name=ERF2; Synonyms=ERF-2;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND TISSUE
RP   SPECIFICITY.
RC   STRAIN=cv. Bright Yellow 4; TISSUE=Leaf;
RX   MEDLINE=95276459; PubMed=7756828; DOI=10.1105/tpc.7.2.173;
RA   Ohme-Takagi M., Shinshi H.;
RT   "Ethylene-inducible DNA binding proteins that interact with an
RT   ethylene responsive element.";
RL   Plant Cell 7:173-182(1995).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RX   DOI=10.1046/j.1365-313x.1998.00243.x;
RA   Suzuki K., Suzuki N., Ohme-Takagi M., Shinshi H.;
RT   "Immediate early induction of mRNAs for ethylene-responsive
RT   transcription factors in tobacco leaf strips after cutting.";
RL   Plant J. 15:657-665(1998).
RN   [3]
RP   FUNCTION, AND INDUCTION.
RX   MEDLINE=20117508; PubMed=10652129;
RX   DOI=10.1046/j.1365-313X.1999.00634.x;
RA   Yamamoto S., Suzuki K., Shinshi H.;
RT   "Elicitor-responsive, ethylene-independent activation of GCC box-
RT   mediated transcription that is regulated by both protein
RT   phosphorylation and dephosphorylation in cultured tobacco cells.";
RL   Plant J. 20:571-579(1999).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   MEDLINE=20252856; PubMed=10792818;
RX   DOI=10.1046/j.1365-313x.2000.00709.x;
RA   Ohta M., Ohme-Takagi M., Shinshi H.;
RT   "Three ethylene-responsive transcription factors in tobacco with
RT   distinct transactivation functions.";
RL   Plant J. 22:29-38(2000).
RN   [5]
RP   INDUCTION.
RX   MEDLINE=22084990; PubMed=12090623; DOI=10.1023/A:1015553232309;
RA   Nishiuchi T., Suzuki K., Kitajima S., Sato F., Shinshi H.;
RT   "Wounding activates immediate early transcription of genes for ERFs in
RT   tobacco plants.";
RL   Plant Mol. Biol. 49:473-482(2002).
CC   -!- FUNCTION: Acts as a transcriptional activator and may be involved
CC       in disease resistance pathways. Binds to the GCC-box pathogenesis-
CC       related promoter element. Involved in the regulation of gene
CC       expression by stress factors and by components of stress signal
CC       transduction pathways mediated by ethylene, that seems to depend
CC       on a protein kinase cascade, and to be influenced by methyl-
CC       jasmonate.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Expressed in roots and at low levels in buds
CC       and leaves. Not expressed in cell culture.
CC   -!- INDUCTION: Induced by ethephon (ethylene-releasing compound) in
CC       buds and to a lower extent in leaves. Strongly induced by
CC       cycloheximide and mechanical stimuli. Wounding leads to a both
CC       local and systemic expression, independently of ethylene, and
CC       through a de-novo-protein-synthesis-independent regulation. Wound
CC       induction is reduced by methyl-jasmonate. Induction by purified
CC       xylanase from Trichoderma viride (TvX) and another elicitor from
CC       Phytophthora infestans (PiE), that appears to be mediated by both
CC       protein kinases and protein phosphatases.
CC   -!- DOMAIN: The AP2/ERF domain binds specifically to the 5'-GCCGCC-3'
CC       motif. The affinity of this binding is higher if the seventh
CC       amino-acid of this domain is basic (By similarity).
CC   -!- SIMILARITY: Belongs to the ethylene-response factor family. Class
CC       1 subfamily.
CC   -!- SIMILARITY: Contains 1 AP2/ERF DNA-binding domain.
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DR   EMBL; D38126; BAA07324.1; -; mRNA.
DR   PIR; T02590; T02590.
DR   HSSP; O80337; 2GCC.
DR   SMR; Q40479; 97-159.
DR   TRANSFAC; T02654; -.
DR   InterPro; IPR001471; TF_ERF.
DR   Pfam; PF00847; AP2; 1.
DR   PRINTS; PR00367; ETHRSPELEMNT.
DR   ProDom; PD001423; TF_ERF; 1.
DR   SMART; SM00380; AP2; 1.
DR   PROSITE; PS51032; AP2_ERF; 1.
KW   Activator; DNA-binding; Ethylene signaling pathway; Nuclear protein;
KW   Plant defense; Transcription; Transcription regulation.
FT   CHAIN         1    233       Ethylene-responsive transcription factor
FT                                2.
FT                                /FTId=PRO_0000112548.
FT   DNA_BIND     98    156       AP2/ERF.
SQ   SEQUENCE   233 AA;  25563 MW;  6CD16783582C0CB5 CRC64;
     MYQPISTELP PTSFSSLMPC LTDTWGDLPL KVDDSEDMVI YGLLSDALTA GWTPFNLTST
     EIKAEPREEI EPATIPVPSV APPAETTTAQ AVVPKGRHYR GVRQRPWGKF AAEIRDPAKN
     GARVWLGTYE TAEEAALAYD KAAYRMRGSK ALLNFPHRIG LNEPEPVRLT AKRRSPEPAS
     SSISSALENG SPKRRRKAVA AKKAELEVQS RSNAMQVGCQ MEQFPVGEQL LVS
//