RID=1168928749-23398-37444222311.BLASTQ1, Contig84

BLASTX 2.2.15 [Oct-15-2006]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. RID: 1168928749-23398-37444222311.BLASTQ1 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples 4,458,890 sequences; 1,532,996,420 total letters

If you have any problems or questions with the results of this search
please refer to the BLAST FAQs Taxonomy reports

Query=  Contig84
Length=680

Distribution of 12 Blast Hits on the Query Sequence

                                                                   Score     E
Sequences producing significant alignments:                       (Bits)  Value

gi|15235569|ref|NP_193953.1|  ISU1 [Arabidopsis thaliana] >gi|...   253    6e-67 
gi|21595581|gb|AAM66114.1|  nifU-like protein [Arabidopsis thalia   252    1e-66 
gi|115439007|ref|NP_001043783.1|  Os01g0662600 [Oryza sativa (...   236    6e-62 
gi|15233385|ref|NP_192317.1|  ATISU3/ISU3; structural molecule...   234    2e-61 
gi|115465511|ref|NP_001056355.1|  Os05g0568100 [Oryza sativa (...   232    1e-60 
gi|16798388|gb|AAL29442.1|AF428267_1  iron-sulfur cluster asse...   219    1e-56 
gi|15232028|ref|NP_186751.1|  ATISU2/ISU2; structural molecule...   218    2e-56 
gi|82937835|emb|CAI29444.1|  Fe-S scaffold protein 3 [Arabidopsis   218    2e-56 
gi|116000506|emb|CAL50186.1|  Predicted lipase/calmodulin-bind...   205    1e-52
gi|116060490|emb|CAL55826.1|  Regucalcin gene promoter region-...  32.3    1.7  
gi|22328334|ref|NP_680590.1|  nucleic acid binding / zinc ion bin  30.8    4.9   
gi|79579715|ref|NP_680579.2|  ubiquitin-protein ligase/ zinc i...  30.0    8.3

Alignments

 

>gi|15235569|ref|NP_193953.1|  ISU1 [Arabidopsis thaliana]
 gi|13605507|gb|AAK32747.1|AF361579_1  AT4g22220/T10I14_50 [Arabidopsis thaliana]
 gi|2832672|emb|CAA16772.1|  nifU-like protein [Arabidopsis thaliana]
 gi|7269067|emb|CAB79177.1|  nifU-like protein [Arabidopsis thaliana]
 gi|19548021|gb|AAL87374.1|  AT4g22220/T10I14_50 [Arabidopsis thaliana]
 gi|82937831|emb|CAI29442.1|  Fe-S scaffold protein 1 [Arabidopsis thaliana]
Length=167

 Score =  253 bits (645),  Expect = 6e-67
 Identities = 127/167 (76%), Positives = 145/167 (86%), Gaps = 1/167 (0%)
 Frame = -3

Query  633  MLRLVSKRLLGLASREFPPRPVQILSRLSHENVIDHYNNPRNVGSFDKNDPNVGTGLVGA  454
            ML+  +K+ LGL SR+  P  V IL R  HENVIDHY+NPRNVGSFDKNDPNVGTGLVGA
Sbjct  2    MLKQAAKKALGLTSRQSTPWSVGIL-RTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGA  60

Query  453  PACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGKSMEEVVTIKNTEI  274
            PACGDVMKLQIK+D+++G+IVDA FKTFGCGSAIASSSVATEWVKGK+ME+V+TIKNTEI
Sbjct  61   PACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEI  120

Query  273  AKHLALPPVKLHCSMLAEDAIKAAVKDVEAKRAKLNGSSNAAPIEKA  133
            AKHL+LPPVKLHCSMLAEDAIKAAVKD + KR K NG++ A    +A
Sbjct  121  AKHLSLPPVKLHCSMLAEDAIKAAVKDYKEKRVKTNGAAAAGETTQA  167


>gi|21595581|gb|AAM66114.1|  nifU-like protein [Arabidopsis thaliana]
Length=166

 Score =  252 bits (643),  Expect = 1e-66
 Identities = 126/167 (75%), Positives = 144/167 (86%), Gaps = 1/167 (0%)
 Frame = -3

Query  633  MLRLVSKRLLGLASREFPPRPVQILSRLSHENVIDHYNNPRNVGSFDKNDPNVGTGLVGA  454
            ML+  +K+ LGL SR+  P  V I  R  HENVIDHY+NPRNVGSFDKNDPNVGTGLVGA
Sbjct  1    MLKQAAKKALGLTSRQSTPWSVSIF-RTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGA  59

Query  453  PACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGKSMEEVVTIKNTEI  274
            PACGDVMKLQIK+D+++G+IVDA FKTFGCGSAIASSSVATEWVKGK+ME+V+TIKNTEI
Sbjct  60   PACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEI  119

Query  273  AKHLALPPVKLHCSMLAEDAIKAAVKDVEAKRAKLNGSSNAAPIEKA  133
            AKHL+LPPVKLHCSMLAEDAIKAAVKD + KR K NG++ A    +A
Sbjct  120  AKHLSLPPVKLHCSMLAEDAIKAAVKDYKEKRVKTNGAAAAGETTQA  166


>gi|115439007|ref|NP_001043783.1|  Os01g0662600 [Oryza sativa (japonica cultivar-group)]
 gi|20521223|dbj|BAB91740.1|  putative iron-sulfur cofactor synthesis protein iscU [Oryza sativa 
(japonica cultivar-group)]
 gi|113533314|dbj|BAF05697.1|  Os01g0662600 [Oryza sativa (japonica cultivar-group)]
Length=171

 Score =  236 bits (602),  Expect = 6e-62
 Identities = 125/175 (71%), Positives = 139/175 (79%), Gaps = 9/175 (5%)
 Frame = -3

Query  633  MLRLVSKRLLGLASREF----PPRPVQIL-SRLSHENVIDHYNNPRNVGSFDKNDPNVGT  469
            MLR   +RLL    R         PV +  ++  HE V+DHY NPRNVGSF+ +DP+VGT
Sbjct  1    MLRAGGRRLLAPGLRRVLGGGAAAPVAVGGAKAYHERVVDHYENPRNVGSFENDDPSVGT  60

Query  468  GLVGAPACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGKSMEEVVTI  289
            GLVGAPACGDVMKLQI++D+ SGKIVDACFKTFGCGSAIASSSVATEWVKGK MEEVVTI
Sbjct  61   GLVGAPACGDVMKLQIRVDESSGKIVDACFKTFGCGSAIASSSVATEWVKGKQMEEVVTI  120

Query  288  KNTEIAKHLALPPVKLHCSMLAEDAIKAAVKDVEAKRAKLNGSSNAAPIEKAADA  124
            KNTEIAKHL+LPPVKLHCSMLAEDAIKAAVKD EAK+AKL         EKAA+A
Sbjct  121  KNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKKAKLAQKGE----EKAAEA  171


>gi|15233385|ref|NP_192317.1|  ATISU3/ISU3; structural molecule [Arabidopsis thaliana]
 gi|3377840|gb|AAC28213.1|  contains similarity to E. coli nitrogen fixation NIFU protein 
(GB:AE000339) [Arabidopsis thaliana]
 gi|7267164|emb|CAB77876.1|  putative NifU-like metallocluster assembly factor [Arabidopsis 
thaliana]
 gi|67633734|gb|AAY78791.1|  putative iron-sulfur cluster assembly complex protein [Arabidopsis 
thaliana]
 gi|82937833|emb|CAI29443.1|  Fe-S scaffold protein 2 [Arabidopsis thaliana]
Length=171

 Score =  234 bits (597),  Expect = 2e-61
 Identities = 119/160 (74%), Positives = 138/160 (86%), Gaps = 4/160 (2%)
 Frame = -3

Query  633  MLRLVSKR-LLGLASREFPPRPVQILSRLSHENVIDHYNNPRNVGSFDKNDPNVGTGLVG  457
            MLR  +KR  LGLAS+   P P  ++SRL H NVIDHY+NPRNVGSFDKNDPNVGTGLVG
Sbjct  1    MLRQTTKRAFLGLASQN--PTPFPVVSRLYHPNVIDHYDNPRNVGSFDKNDPNVGTGLVG  58

Query  456  APACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGKSMEEVVTIKNTE  277
            AP CGDVMKLQ+K D  SG+I+DA FKTFGCGSAIA+SSVATEWVKGKS+EEV+TIKN++
Sbjct  59   APQCGDVMKLQVKFDG-SGQIIDAKFKTFGCGSAIAASSVATEWVKGKSVEEVLTIKNSQ  117

Query  276  IAKHLALPPVKLHCSMLAEDAIKAAVKDVEAKRAKLNGSS  157
            IAKHL+LPPVKLHCSMLAEDAIKAA+K+ + K+ K NG +
Sbjct  118  IAKHLSLPPVKLHCSMLAEDAIKAAIKNYKEKQDKANGET  157


>gi|115465511|ref|NP_001056355.1|  Os05g0568100 [Oryza sativa (japonica cultivar-group)]
 gi|51038122|gb|AAT93925.1|  putative iron-sulfur cluster assembly complex protein [Oryza 
sativa (japonica cultivar-group)]
 gi|51854290|gb|AAU10671.1|  putative nifU-like protein [Oryza sativa (japonica cultivar-group)]
 gi|113579906|dbj|BAF18269.1|  Os05g0568100 [Oryza sativa (japonica cultivar-group)]
Length=172

 Score =  232 bits (591),  Expect = 1e-60
 Identities = 117/168 (69%), Positives = 137/168 (81%), Gaps = 12/168 (7%)
 Frame = -3

Query  633  MLRLVSKRLLGL-----------ASREFPPRPVQILSRLS-HENVIDHYNNPRNVGSFDK  490
            MLR   KRLLG+           A+       V ++ R   HE V+DHY+NPRNVG+FDK
Sbjct  1    MLRAAGKRLLGVGLRPAGGGAGEAAAAAAASAVAVVRRRGYHERVVDHYDNPRNVGTFDK  60

Query  489  NDPNVGTGLVGAPACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGKS  310
            +DP+VGTGLVGAPACGDVMKLQI++D+ESG+IVDACFKTFGCGSAIASSSVA+EWVKGK 
Sbjct  61   DDPDVGTGLVGAPACGDVMKLQIRVDEESGRIVDACFKTFGCGSAIASSSVASEWVKGKQ  120

Query  309  MEEVVTIKNTEIAKHLALPPVKLHCSMLAEDAIKAAVKDVEAKRAKLN  166
            ME+  +IKN+EIAKHL+LPPVKLHCSMLAEDAIKAAVKD EAK+AKL+
Sbjct  121  MEDAASIKNSEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYEAKKAKLD  168


>gi|16798388|gb|AAL29442.1|AF428267_1  iron-sulfur cluster assembly protein IscU [Chlamydomonas reinhardtii]
Length=163

 Score =  219 bits (557),  Expect = 1e-56
 Identities = 111/156 (71%), Positives = 128/156 (82%), Gaps = 1/156 (0%)
 Frame = -3

Query  639  ARMLRLVSKRLLGLASREFPPRPVQILSRLSHENVIDHYNNPRNVGSFDKNDPNVGTGLV  460
            A +L   +  L   A    P    Q+  RL H+NV++HY  PRNVGSFDK+DP+VGTGLV
Sbjct  3    ASLLPSAASALSAPAVGALPLLAQQLGRRLYHKNVVEHYERPRNVGSFDKSDPDVGTGLV  62

Query  459  GAPACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGKSMEEVVTIKNT  280
            GAPACGDVMKLQIK+D E G IVDA FKTFGCGSAIASSSVATEWVKGKS++EV+ IKN+
Sbjct  63   GAPACGDVMKLQIKVD-EQGNIVDARFKTFGCGSAIASSSVATEWVKGKSLDEVLKIKNS  121

Query  279  EIAKHLALPPVKLHCSMLAEDAIKAAVKDVEAKRAK  172
            +IAKHL+LPPVKLHCSMLAEDAIKAAVKD++AKR K
Sbjct  122  DIAKHLSLPPVKLHCSMLAEDAIKAAVKDIQAKRTK  157


>gi|15232028|ref|NP_186751.1|  ATISU2/ISU2; structural molecule [Arabidopsis thaliana]
 gi|6714486|gb|AAF26172.1|AC008261_29  unknown protein [Arabidopsis thaliana]
 gi|67633616|gb|AAY78732.1|  putative iron-sulfur cluster assembly complex protein [Arabidopsis 
thaliana]
Length=163

 Score =  218 bits (555),  Expect = 2e-56
 Identities = 110/160 (68%), Positives = 134/160 (83%), Gaps = 7/160 (4%)
 Frame = -3

Query  633  MLRLVSKRL-LGLASREFPPRPVQILSRLSHENVIDHYNNPRNVGSFDKNDPNVGTGLVG  457
            MLR  S++  LGL +      P+ +  RL HENVIDH+ NPRNVGSF++NDPNVGTGLVG
Sbjct  3    MLRQTSRKAYLGLQAS-----PLGLGRRLYHENVIDHFENPRNVGSFNRNDPNVGTGLVG  57

Query  456  APACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGKSMEEVVTIKNTE  277
            APACGD+M LQIK+DD SG+I+D  FKTFGCGSAIASSSVA+EW+KGK+++EV+TIKN E
Sbjct  58   APACGDLMSLQIKVDD-SGQIIDTRFKTFGCGSAIASSSVASEWIKGKTLDEVMTIKNAE  116

Query  276  IAKHLALPPVKLHCSMLAEDAIKAAVKDVEAKRAKLNGSS  157
            IAKHL LPPVKLHCSMLAEDAIK+AV+D + K+AK N ++
Sbjct  117  IAKHLRLPPVKLHCSMLAEDAIKSAVRDYKEKQAKTNAAA  156


>gi|82937835|emb|CAI29444.1|  Fe-S scaffold protein 3 [Arabidopsis thaliana]
Length=162

 Score =  218 bits (555),  Expect = 2e-56
 Identities = 110/160 (68%), Positives = 134/160 (83%), Gaps = 7/160 (4%)
 Frame = -3

Query  633  MLRLVSKRL-LGLASREFPPRPVQILSRLSHENVIDHYNNPRNVGSFDKNDPNVGTGLVG  457
            MLR  S++  LGL +      P+ +  RL HENVIDH+ NPRNVGSF++NDPNVGTGLVG
Sbjct  2    MLRQTSRKAYLGLQAS-----PLGLGRRLYHENVIDHFENPRNVGSFNRNDPNVGTGLVG  56

Query  456  APACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGKSMEEVVTIKNTE  277
            APACGD+M LQIK+DD SG+I+D  FKTFGCGSAIASSSVA+EW+KGK+++EV+TIKN E
Sbjct  57   APACGDLMSLQIKVDD-SGQIIDTRFKTFGCGSAIASSSVASEWIKGKTLDEVMTIKNAE  115

Query  276  IAKHLALPPVKLHCSMLAEDAIKAAVKDVEAKRAKLNGSS  157
            IAKHL LPPVKLHCSMLAEDAIK+AV+D + K+AK N ++
Sbjct  116  IAKHLRLPPVKLHCSMLAEDAIKSAVRDYKEKQAKTNAAA  155


>gi|116000506|emb|CAL50186.1|  Predicted lipase/calmodulin-binding heat-shock protein (ISS) 
[Ostreococcus tauri]
Length=658

 Score =  205 bits (522),  Expect = 1e-52
 Identities = 99/126 (78%), Positives = 114/126 (90%), Gaps = 1/126 (0%)
 Frame = -3

Query  555  RLSHENVIDHYNNPRNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKIDDESGKIVDACFK  376
            RL+  +V++H++NPRNVGSFDK D NVGTG+VGAPACGDVMKLQIKID+E G IVD+CFK
Sbjct  525  RLTPSSVVEHFDNPRNVGSFDKKDMNVGTGIVGAPACGDVMKLQIKIDEE-GNIVDSCFK  583

Query  375  TFGCGSAIASSSVATEWVKGKSMEEVVTIKNTEIAKHLALPPVKLHCSMLAEDAIKAAVK  196
            TFGCGSAIASSSVATEW+KG  M+E VTIKN +IAKHL LPPVKLHCSMLAEDAI++AV 
Sbjct  584  TFGCGSAIASSSVATEWIKGTHMDEAVTIKNQDIAKHLNLPPVKLHCSMLAEDAIRSAVA  643

Query  195  DVEAKR  178
            DV+AKR
Sbjct  644  DVKAKR  649


>gi|116060490|emb|CAL55826.1|  Regucalcin gene promoter region-related protein (RGPR) (ISS) 
[Ostreococcus tauri]
Length=1112

 Score = 32.3 bits (72),  Expect = 1.7
 Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 0/43 (0%)
 Frame = -3

Query  513  RNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKIDDESGKIVDA  385
            R+V S DKN P V   LVG  A     +LQ  +  ++G++ DA
Sbjct  536  RSVRSLDKNSPEVNVVLVGTLAAQMEHRLQNALKGKAGRLADA  578


>gi|22328334|ref|NP_680590.1|  nucleic acid binding / zinc ion binding [Arabidopsis thaliana]
Length=230

 Score = 30.8 bits (68),  Expect = 4.9
 Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 0/53 (0%)
 Frame = -3

Query  471  TGLVGAPACGDVMKLQIKIDDESGKIVDACFKTFGCGSAIASSSVATEWVKGK  313
            +G+  +  C DV+ L+ K+  +S      C K+F C  A+         +KGK
Sbjct  67   SGMEKSTTCSDVVALRSKLQSKSSHRCQICGKSFECYQALGGHRRLHRPIKGK  119


>gi|79579715|ref|NP_680579.2|  ubiquitin-protein ligase/ zinc ion binding [Arabidopsis thaliana]
 gi|52354369|gb|AAU44505.1|  hypothetical protein AT4G03965 [Arabidopsis thaliana]
 gi|55740645|gb|AAV63915.1|  hypothetical protein At4g03965 [Arabidopsis thaliana]
Length=209

 Score = 30.0 bits (66),  Expect = 8.3
 Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 4/59 (6%)
 Frame = -3

Query  261  ALPPVKLHCSMLAED-AIKAAVKDVEAKRAKLNGSSNAAPIEKAADA*CLCICRGRKSG  88
            +L P+K+    L E+ A +  V++ E +R   + SS    +    D+ C C+C GRK G
Sbjct  115  SLTPLKVSLMRLLEETAERVVVEENETERVTASASSTVRGVNN--DSVC-CVCMGRKKG  170

  Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding 
environmental samples
    Posted date:  Jan 15, 2007  4:53 AM
  Number of letters in database: 101,054,579
  Number of sequences in database:  283,349
Lambda     K      H
   0.318    0.134    0.401 
Gapped
Lambda     K      H
   0.267   0.0410    0.140 
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 283349
Number of Hits to DB: 466
Number of extensions: 8
Number of successful extensions: 0
Number of sequences better than 10: 0
Number of HSP's better than 10 without gapping: 0
Number of HSP's gapped: 0
Number of HSP's successfully gapped: 0
Length of query: 680
Length of database: 101054579
Length adjustment: 109
Effective length of query: 571
Effective length of database: 70169538
Effective search space: 8209835946
Effective search space used: 8209835946
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 65 (29.6 bits)