Stress gene

Primer List of Genes and Proteins

14-3-3 PROTEIN PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	GCTTATGAGGCTGCCACTTC	488	20	59.98	55.00	AGGTCCACAAGGTGAGGTTG	734	20	60.00	55.00	247
2	CCTCATTCCATCTTCCTCCA	358	20	60.00	50.00	00GAAGTGGCAGCCTCATAAGC	507	20	59.98	55.00	150
3	AAGCTCAGATTTGCCTCCAA	514	20	59.96	45.00	AGGTCCACAAGGTGAGGTTG	734	20	60.00	55.00	221
4	GCCTCCAACTCACCCAATTA	526	20	59.93	50.00	AGGTCCACAAGGTGAGGTTG	734	20	60.00	55.00	209
5	CCTCATTCCATCTTCCTCCA	358	20	60.00	50.00	GGCCAAGTCTAATTGGGTGA	554	20	59.93	50.00	197
6	AGATTGGGGAGGAGAGGAAA	88418	20	60.01	50.00	TTGCTTCCTCAAAAGCCTGT	88618	20	59.99	45.00	201
7	TTGGGGAGGAGAGGAAATCT	88421	20	60.01	50.00	TTGCTTCCTCAAAAGCCTGT	88618	20	59.99	45.00	198
8	GCTTATGAGGCTGCCACTTC	488	20	59.98	55.00	AGGTCCACAAGGTGAGGTTG	734	20	60.00	55.00	247
9	CCTCATTCCATCTTCCTCCA	358	20	60.00	50.00	GAAGTGGCAGCCTCATAAGC	507	20	59.98	55.00	150
10	TTGTTGAATTGAGCCAGCAG	83443	20	59.99	45.00	TCCACATCAAGCTGAGCAAC	83655	20	59.99	50.00	213

DEHYDRIN PROTEIN PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	GAGAAATTGCCAGGTGAGGA	159	20	60.20	50.00	TCTTGTCCATGATCCCCTTC	314	20	59.86	54.00	156
2	GAGAAATTGCCAGGTGAGGA	159	20	60.20	50.00	TGTCCATGATCCCCTTCTTC	311	20	59.86	50.00	153
3	ATTGCCAGGTGAGGAAGAGA	164	20	59.80	50.00	TCTTGTCCATGATCCCCTTC	314	20	59.86	50.00	151
4	CCAGGTGAGGAAACAGAGGA	111	20	60.23	55.00	TCTTGTCCATGATCCCCTTC	314	20	59.86	50.00	204
5	AAATTGCCAGGTGAGGAAGA	162	20	59.67	45.00	TCTTGTCCATGATCCCCTTC	314	20	59.86	50.00	153

DREB GENE PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	GGAGCCAAACAAGAAATCCA	901	20	60.05	45.00	AAATCAGCCAAAGCCTGCTA	1055	20	59.98	45.00	155
2	GGAGCCAAACAAGAAATCCA	901	20	60.05	45.00	TCTCAGCAACCCATTTACCC	1076	20	59.93	50.00	176
3	GGAGCCAAACAAGAAATCCA	901	20	60.05	45.00	TTACCCCAAATCAGCCAAAG	1062	20	59.93	45.00	162
4	AACTCCGGTAAGTGGGTGTG	812	20	59.88	55.00	AAATCAGCCAAAGCCTGCTA	1055	20	59.98	45.00	244
5	GGGTAAATGGGTTGCTGAGA	1057	20	59.93	50.00	CCATCTCAGCGGTTTGAAAT	1256	20	60.07	45.00	200

HSP (Heat Shock Protein) PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	GAGGAGTTGCTTTGGCAGTC	2534	20	60.00	55.00	CAGCGCCTGCATCTATGTAA	2719	20	60.00	50.00	186
2	GTCACTGATGCTGCTTTGGA	2551	20	59.99	50.00	CAGCGCCTGCATCTATGTAA	2719	20	60.00	50.00	169
3	TCACTGATGCTGCTTTGGAC	2552	20	59.99	50.00	CAGCGCCTGCATCTATGTAA	2719	20	60.00	50.00	168
4	AATCGTCGCAGAAGTCTCGT	6547	20	60.02	50.00	CAAGCTTTCCTGCTTGTTCC	6792	20	59.99	50.00	246
5	AATCGTCGCAGAAGTCTCGT	6547	20	60.02	50.00	AAGCTTTCCTGCTTGTTCCA	6791	20	59.99	45.00	245

LEA PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	CTGCTGGTGGAACTGGGTAT	652	20	59.99	55.00	ATTCTCCGCCATTCCTTTCT	824	20	60.04	45.00	173
2	TGACGCTGCTCGTCATAATC	2469	20	59.98	50.00	GGCTTCTGGTTTGAATTGGA	2639	20	60.05	45.00	171
3	CACCCTTGCTCATCCTTCAT	4487	20	60.07	50.00	GGCCACTTTCACTCTTCTCG	4647	20	59.99	55.00	161
4	GGCATGGCATATGAGAGGTT	503	20	59.92	50.00	ATACCCAGTTCCACCAGCAG	671	20	59.99	55.00	169
5	TAATGGAGTGGCATGGCATA	494	20	59.91	45.00	ATACCCAGTTCCACCAGCAG	671	20	59.99	55.00	178

LTP PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	GCTAGGGCTCGAAGTTGTTG	81770	20	60.02	55.00	GCTGCTTCTTCCCAAGTCAC	81954	20	60.00	55.00	185
2	CTAGGGCTCGAAGTTGTTGC	81771	20	60.02	55.00	GCTGCTTCTTCCCAAGTCAC	81954	20	60.00	55.00	184
3	AAAGGCAAAGCAAAAGCAAA	3858	20	60.00	35.00	CTCTTCACTCCACCACAGCA	4092	20	60.02	55.00	235
4	AAAAGGCAAAGCAAAAGCAA	3857	20	60.00	35.00	CTCTTCACTCCACCACAGCA	4092	20	60.02	55.00	236
5	AAAAAGGCAAAGCAAAAGCA	3856	20	60.00	35.00	CTCTTCACTCCACCACAGCA	4092	20	60.02	55.00	237

MAPKINASE PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	GCACTTTGCCACCCATACTT	59349	20	60.00	50.00	GAGAATCGGGTCAGGATTGA	59510	20	60.01	50.00	162
2	AAATTCCCCGTATGGGTCTC	5037	20	60.02	50.00	GAACCCGAGTCGTTGTTGTT	5189	20	60.01	50.00	153
3	CGTGACTGATCTGCTTGGAA	9317	20	59.98	50.00	AAGCGATCAAACGCTGAAGT	9486	20	60.02	45.00	170
4	TGGAGGGAAGAATGGAGATG	26852	20	60.00	50.00	TTCCTTACCACCCTTCGTTG	27076	20	59.96	50.00	225
5	GGAGCAAATGGCAGATGATT	62783	20	60.04	45.00	AGGGACGGTATGAGCAACAC	62941	20	60.00	55.00	159

SERK PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	CTGGACAGCTCGTAATGCAA	13556	20	60.01	50.00	CTTAAGTCGGCCGAGAGTTG	13734	20	60.01	55.00	179
2	GGAGGAGGAGGAGAAAATGG	11371	20	59.98	45.00	ACTGTCAAAATTGCGGTTCC	11616	20	60.01	55.00	246
3	CAGCAGGCACATCAAAGAAA	9190	20	60.02	55.00	CTCCAACTCCTGTGCAGTCA	9348	20	59.99	45.00	159
4	AGAAGAAAACCGCGAGAACA	15952	20	59.99	45.00	TTGAAACTGCAGCTCACCAC	16191	20	60.03	50.00	240
5	CTGGACAGCTCGTAATGCAA	13556	20	60.01	50.00	GGCCGAGAGTTGATGGAATA	13726	20	60.04	50.00	171

WRKY PRIMERS

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Sl.NO	Forward	START	Length	TM	GC	Reverse	START	Length	TM	GC	Product Size
1	ACAGCTCCAGCAACGAAGAT	11877	20	60.02	50.00	GGTGACGGGTTGTCTCTTGT	12093	20	60.01	55.00	217
2	TTCTGCAGGCTCGTTATCCT	76136	20	59.99	45.00	CAGCAAAGCATTCCAGAACA	76359	20	59.98	50.00	224
3	CAGCAAAGCATTCCAGAACA	73665	20	59.99	45.00	TTCTGCAGGCTCGTTATCCT	73888	20	59.98	50.00	224
4	AATTGCAGTCACCAACCACA	600	20	60.01	45.00	AGTTGAAGCCATCACCAACC	800	20	59.97	50.00	201
5	CAATTGCAGTCACCAACCAC	599	20	60.01	50.00	AGTTGAAGCCATCACCAACC	800	20	59.97	50.00	202