Sl.NO Forward START Length TM GC Reverse START Length TM GC Product Size 1 GAGAAATTGCCAGGTGAGGA 159 20 60.20 50.00 TCTTGTCCATGATCCCCTTC 314 20 59.86 54.00 156 2 GAGAAATTGCCAGGTGAGGA 159 20 60.20 50.00 TGTCCATGATCCCCTTCTTC 311 20 59.86 50.00 153 3 ATTGCCAGGTGAGGAAGAGA 164 20 59.80 50.00 TCTTGTCCATGATCCCCTTC 314 20 59.86 50.00 151 4 CCAGGTGAGGAAACAGAGGA 111 20 60.23 55.00 TCTTGTCCATGATCCCCTTC 314 20 59.86 50.00 204 5 AAATTGCCAGGTGAGGAAGA 162 20 59.67 45.00 TCTTGTCCATGATCCCCTTC 314 20 59.86 50.00 153

Sl.NO Forward START Length TM GC Reverse START Length TM GC Product Size 1 GGAGCCAAACAAGAAATCCA 901 20 60.05 45.00 AAATCAGCCAAAGCCTGCTA 1055 20 59.98 45.00 155 2 GGAGCCAAACAAGAAATCCA 901 20 60.05 45.00 TCTCAGCAACCCATTTACCC 1076 20 59.93 50.00 176 3 GGAGCCAAACAAGAAATCCA 901 20 60.05 45.00 TTACCCCAAATCAGCCAAAG 1062 20 59.93 45.00 162 4 AACTCCGGTAAGTGGGTGTG 812 20 59.88 55.00 AAATCAGCCAAAGCCTGCTA 1055 20 59.98 45.00 244 5 GGGTAAATGGGTTGCTGAGA 1057 20 59.93 50.00 CCATCTCAGCGGTTTGAAAT 1256 20 60.07 45.00 200