The Computational analysis of Cocoa EST - SSR by SSR Primer (Robinson et al . 2004 )

SSR type Total Percentage Dinucleotide 10 21.74% Trinucleotide 27 58.70% Tetranucleotie 6 13.04% Pentanucleotide 3 6.50% Total number of sequences analyzed: 6581 Total number of SSRs found in sequences: 46 The trinucleotide 'CTG' has repeated 7 (15.22%) of the total SSR

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Sl.NO Accession NO SSR Repeat Type SSR Repeats Start End Sequence Length 1 CA797290 trinucleotide CTGCTGCTGCTGCTGCTGCT 438 457 20 2 CA797461 trinucleotide CTGCTGCTGCTGCTGCTGCT 438 457 20 3 CA797383 trinucleotide CTGCTGCTGCTGCTGCTGCT 440 459 20 4 CA797237 trinucleotide CTGCTGCTGCTGCTGCTGCT 438 457 20 5 CA797340 trinucleotide CTGCTGCTGCTGCTGCTGCT 438 457 20 6 CA798049 trinucleotide CTGCTGCTGTTGCTGCTGCTGCT 80 102 23 7 CA795268 trinucleotide CTGCTGCTGTTGCTGCTGCTGCT 118 140 23 8 CA798214 trinucleotide CTTCTTCTTCCTCTTCTTCTTCTTCTTCTTCTT 89 121 33 9 CA798038 trinucleotide CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 202 232 31 10 CA797995 trinucleotide GAAGAAGAAGAAGAAGAAGAAG 90 111 22 11 CA795200 trinucleotide GAAGAAGAAGAAGAAGAAGAAGAAGA 167 192 26 12 CA794352 trinucleotide GAAGAAGAAGAAGAAGAAGAAGAAGA 167 192 26 13 CA795199 trinucleotide GAAGAAGAAGAAGAAGAAGAAGAAGA 167 192 26 14 CA798544 trinucleotide GAAGAAGAAGAAGAAGAAGAAGAAGA 115 140 26 15 CA795401 trinucleotide GCAGCNNCAGCAGCAGCAGC 78 97 20 16 CF974443 tetranucleotide GTATGTATGTATGTATGTAT 56 75 20 17 CF974306 tetranucleotide GTATGTATGTATGTATGTAT 60 79 20 18 CA798018 dinucleotide TATATATATATATATATATATA 97 118 22 19 CA798030 dinucleotide TATATATATATATATATATATAT 105 127 23 20 CF974239 trinucleotide TCATCATCATCATCATCATCATCATCA 94 120 27
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