The Computational analysis of Cocoa EST - SSR by SSR Primer (Robinson et al . 2004 )

SSR type Total Percentage Dinucleotide 10 21.74% Trinucleotide 27 58.70% Tetranucleotie 6 13.04% Pentanucleotide 3 6.50% Total number of sequences analyzed: 6581 Total number of SSRs found in sequences: 46 The trinucleotide 'CTG' has repeated 7 (15.22%) of the total SSR

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Sl.NO Accession NO SSR Repeat Type SSR Repeats Start End Sequence Length 1 CA797713 trinucleotide AACAACAACAACAACAACAACAA 134 156 23 2 CA797758 trinucleotide AACAACAACAACAACAACAACAA 134 156 23 3 CF972885 trinucleotide AAGAAGAAGAAGAAGAAGAAG 396 416 21 4 CA797368 trinucleotide AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 36 67 32 5 CA798467 trinucleotide AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 77 108 32 6 CA797402 trinucleotide AATAATAATAATACTAATAATAAT 166 189 24 7 CA794473 pentanucleotide ACCAAACCAAACCAAACCAAAC 55 76 22 8 CA794442 pentanucleotide ACCAAACCAAACCAAACCAAAC 55 76 22 9 CF973892 trinucleotide ACCACCACCACCACCATCACCACCA 304 328 25 10 CA797900 dinucleotide AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG 66 97 32 11 CF972800 tetranucleotide ATAAATAAATAAATAAATAA 275 294 20 12 CF972661 tetranucleotide ATAAATAAATAAATAAATAA 275 294 20 13 CF972780 tetranucleotide ATAAATAAATAAATAAATNA 275 294 20 14 CA797706 pentanucleotide ATAACATAACATCACATAACA 259 279 21 15 CA796357 dinucleotide ATATATATATATATATATATAT 64 85 22 16 CA797336 dinucleotide ATATATATATATATATATATAT 64 85 22 17 CA797532 dinucleotide ATATATATATATATATATATAT 64 85 22 18 CA797692 dinucleotide CACACACACACACACACACA 121 140 20 19 CF972909 trinucleotide CATCATCATCATCATCATCATCATCA 261 286 26 20 CA794771 dinucleotide CTCTCTCTCTCTCTCTCTCT 66 85 20
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